What is SameSpots?
SameSpots analyses 2D gel images using an alignment based approach which is proven to generate reproducible results. It includes a gel QC workflow, and the tools to carry out a detailed investigation of your data through to spot picking and reporting.
SameSpots Analysis Workflow
Analyse 2DE, DIGE and secondary stained gel images and get reproducible results.
SpotCheck Gel QC Workflow
Quickly and objectively measure whether a 2D gel meets your lab’s quality requirements.
Using SameSpots, you’ll benefit from:
- speed - typical analysis times of 5 minutes per image
- objectivity – automatic analysis and no editing required in most cases
- statistics - complete data with no missing values
- reproducibility of results across labs
- a built in gel QC workflow
- integration of protein identification data
- a clip gallery to help with the production of posters and publications
The speed to run enough replicates for valid conclusions
The alignment based analysis approach developed for SameSpots has significantly improved the time taken to analyse gels compared to traditional analysis approaches. Typical analysis times are less than 5 minutes per gel, so it is realistic to run enough replicates in your experiment to control for biological variation.
Different users – same answer
The optimised workflow in SameSpots has been designed to require minimal user intervention. SameSpots contains many automatic processes so an objective analysis is achievable and different users in the same lab can get the same results.
100% matching and no missing values
Accurate gel alignment accounts for gel distortions and positions all spots in exactly the same location. Spot detection produces a complete data set as all gels contain the same number of spots, each matched to its corresponding spot on all gels. There are no missing values allowing valid multivariate statistical analysis to be applied. Tests included are Anova p-value, False Discovery Rate (q-values), Principal Components Analysis (PCA), Correlation Analysis and Power Analysis.
SameSpots helps you overcome the challenge of cross-lab reproducibility so you can be confident you are reporting actual biological changes, rather than artefacts of an experimental approach. The HUPO Reproducibility Study proves that protein expression is reproducible across labs and highly reproducible within lab.
Answer to the common question, "Is my lab running gels in a reproducible manner?"
SameSpots has an integrated gel QC workflow called SpotCheck. It allows you to quickly and objectively measure whether a 2D gel meets your lab’s quality requirements.
To get started you need a gold standard. This is used as a baseline to objectively measure the quality of your gels. You can then check your test images against the gold standard to check that gel running is maintained at an acceptable quality.
You get an immediate pass or fail assessment of each image and a colour coded whole image view to show you which spots passed, and which spots failed.
Link protein IDs and spot data so you can see the whole picture
SameSpots supports the integration of MS-based protein identifications (from Mascot or a .csv file). This helps put your 2D results into biological context as you can view the protein IDs alongside the spot measurements from which they originated. You can also use molecular weight markers to help you confirm the accuracy of your IDs.
Convenient capture of software screen shots using the clip gallery
As you work through your analysis you can add images and tables to a clip gallery to help with the production of publications, posters and presentations. They are saved as high resolution .png files (300 dpi) which are ideal for print and you have the option to add some notes for future reference. The clip gallery contents can be exported to a memory stick so they can be accessed at your convenience.
Want to learn more?
For more information on SameSpots and its approach to data analysis, please see the following:
- How SameSpots works: an overview of how analysis is performed, focussing on the most important technical details that differentiate SameSpots from other software
- Frequently-asked questions: answers to many of the most common questions we're asked
- Analysis workflow online tutorial: will guide new users through each step of the SameSpots analysis workflow
- QC workflow online tutorial: will guide new users through the SpotCheck workflow
Finally, if you have any questions that are not covered by these pages, please ask us a question. We aim to respond to all enquiries within 1 working day.